CD34 and type 1 diabetes mellitus: Isolated CD34+ cells (HSPCs) obtained from T1D patients and from healthy controls were cultured using StemSpan SFEMII supplemented with the aforementioned human stem cell growth factors (STFIA medium) and pulsed with PGE2 (10 μM) at 24, 96 h and at 7 days at 37°C 5% CO2.