While investigating the impact of increased SNHG1 in CCA, at first, as evident from Fig. 2a, qRT-PCR findings suggested that the expression of SNHG1 in the small interfering RNA (siRNA)-mediated knockdown group was substantially lowered, in comparison with the si-SC (scramble negative control) group, in the HuCCT1 and RBE cell lines (Fig. 2a). This evidence concerns the gene SNHG1 and cholangiocarcinoma.