Multivariate analysis was also performed with baseline prognostic variables included age, gender, ECOG, PIK3CA, histological grade, clinical stage and lymph node metastasis, which indicated PIK3CAmutation/LGR5+ was a detrimental factor for both DFS and OS (HR = 3.148, 95% CI: 1.987–4.986, p < 0.001; HR = 2.221, 95% CI: 1.221–4.040, p = 0.009, respectively. Here, PIK3CA is linked to metastatic malignant neoplasm in the lymph nodes.