In addition, qRT-PCR and western blot analysis of CPNE1 mRNA and protein expression, respectively, in six NSCLC cell lines and BEAS-2B cells (Fig. 1e) showed that the incidence of high CPNE1 expression was higher in the NSCLC tissues and cell lines than in the normal cell samples. The gene discussed is CPNE1; the disease is non-small cell lung carcinoma.