To further investigate the function of CPNE1 in NSCLC cells, we first established A549 and H1299 cell lines with stable overexpression of CPNE1: this was confirmed by the high level of CPNE1 mRNA and protein expression that was consistently observed in the cells overexpressing CPNE1. CCK-8 and clonogenic assays showed that the growth of cells overexpressing CPNE1 was significantly promoted compared with the control cells. The gene discussed is CPNE1; the disease is non-small cell lung carcinoma.