Finally, we found that MK2206 also reversed the effect of IQUB overexpression on breast cancer cells by clone formation assay and Wound healing assay, which indicated that IQUB could promote proliferation and migration of breast cancer cells through activating Akt/GSK3β/β‐catenin signaling pathway (Figure 6C). The gene discussed is AKT1; the disease is breast carcinoma.