NR1H4 and intrahepatic cholestasis: To determine if phosphorylation of FXR at Y67 is also important for adaptive responses to biliary insults, mice expressing either FXR-WT or Y67F-FXR were challenged with α-naphthyl isothiocyanate (ANIT) (Fig. 3a, b), which induces intrahepatic cholestasis by injuring biliary epithelial cells28,33,34.