To address the expression patterns of NF-L, NF-M, NF-H, and INA in ischemia-affected tissue, we applied double immunofluorescence labeling for NF-L and NF-H as well as NF-M and INA in the mouse model of fMCAO and the rat model of eMCAO at 24 h after ischemia induction. Here, NEFM is linked to ischemia.