NEFL and ischemia: Importantly, the NF-L degradation products smaller than 65 kDa were not detected by the monoclonal mouse-anti-NF-L antibody (DA2) in the western blot (Supplementary Figure S2b), which corresponds with decreased fluorescence intensities in the ischemia-affected tissue, thereby co-localizing with the increased immunosignal of the rabbit NF-L antibody (Supplementary Figure S2c).