In conclusion, our findings reveal that in one individual, the MTB peptide E7/HLA-DR tetramers constructed with different HLA-DRB1 alleles can bind to CD4+ T cells due to sharing the same CDR3 amino-acid sequence or a similar CDR3 structure and function, which suggests that E7-bound CD4+ T cells with different HLA-DRB1 alleles undergo clonal expansion in TB patients. This evidence concerns the gene HLA-DRB1 and tuberculosis.