Using Phos-tag bidimensional gel electrophoresis (2D Phos-tag) and WB (Figure 3), we analyzed nuclear and cytoplasmic extracts from the androgen-dependent PCa cell line LNCaP and from cell lines with different CRPC phenotypes with respect to AR status and transcriptional activity: three AR-positive cell lines (PDB, hypersensitive; MDB, inactive; 22Rv1, ARv7, androgen-independent) and the AR-negative PC3 cell line [25]. This evidence concerns the gene ABR and posterior cortical atrophy.