Sorted CD8+ T cells from PB of healthy donors were cultured in vitro on a plate coated with ICAM-1 Fc chimera to mimic the engagement of ICAM-1-mediated engagement of tumor cells binding to LFA-1 on the surface of CD8+ T cells, in the presence of soluble human recombinant TGFβ2 and anti-CD3. Here, ICAM1 is linked to neoplasm.