HPβCD is known to inhibit migration of human MDA-MB 231 breast cancer cells.(Fig 3B in [72]) [73, 74] To determine whether inhibition of migration is caused by HPβCD depleting cholesterol, as assumed previously, or by it depleting phospholipids, as implicated by the novel genetics results, the published activity from wound healing experiments comparing HPβCD against control was replicated, and complemented with novel activity results comparing HPαCD against control,,both in MDA-MB 231 (ER–) and MCF-7 (ER+) human breast epithelial cell lines. Here, ESR1 is linked to breast cancer.