To study the effects of eEF2K inhibition on AS toxicity, we employed differentiated mouse neuroblastoma N2A cells overexpressing either wild-type AS (ASyn-WT) or the A53T mutant (ASyn-A53T) +/− siRNA mediated eEF2K knockdown (kd), and measured cytotoxicity in these cells. Here, EEF2K is linked to neuroblastoma.