DNM1L and colorectal carcinoma: To directly test DRP1’s requirement in H2O2-mediatedmitochondrial fragmentation in a genetically clean model, we next examinedHCT116 colorectal carcinoma cells with (control) or lacking DRP1 (DRP1 KO).These cells allow a direct interrogation of DRP1’s role, as acomplementary approach to the experiments above in H9c2 and 143B cell lines.While HCT116s showed the expected balance of mitochondrial fission and fusion,H2O2-challenged HCT116s displayed near-totalmitochondrial fragmentation.