Having shown acquisition of anchorage‐independent growth as well as the increased migratory and invasive capacity of HNSCC cells with miR‐9 knockdown and CXCR4‐overexpressing cells in 2D culture, we then went on to investigate these phenotypes in 3D anchorage‐independent condition. This evidence concerns the gene CXCR4 and head and neck squamous cell carcinoma.