The post hoc analyses (n = 3, Bonferroni correct p‐value of 0.0016) showed a significant increase in ATP requirements in patients with ALS if a stoichiometry of NfL:NfM:NfH of 7 : 3 : 2 was to be maintained compared with both control subjects and ALS patients with a Nf isoform stoichiometry of 24 : 2.4 : 1.6 (p < 0.0001 for both comparisons). This evidence concerns the gene NEFM and amyotrophic lateral sclerosis.