Laser imaging of intracellular NF-κβp65 expression of Pan02 tumor cell line which was exposured to 1-MT or non exposured to 1-MT showed that IDO depletion effectively inhibited NF-κβp65 signaling pathway (Fig. 6), that ensured a role of IDO in NF-κβ pathway activation in response to regulatory polarization, the inhibiting of this pathway by 1-MT leads to breakdown tumor tolerance which facilitate modulation of immune responses inside tumor microenvironment. The gene discussed is NFKB1; the disease is neoplasm.