CD4 and neoplasm: On the other hand, the determination of optimal time point to activate T cell kinetic and polarization toward tumor antigens showed that accumulation of intracellular INF-γ+ in CD3+CD4+ cells was increased during 9 hours to reveal 3.15%, but a significant level of INF-γ was detected after 24 hours 6.35% as represented in (Fig. 1C); whereas the accumulation of INF-γ in CD3+CD8+ cells was also showed significant raising after 24 hours 7.65% as seen in (Fig. 1D).