The morphological data from immunofluorescence of MAP2, as well as the protein and mRNA expression data suggest that the 3D primary neuronal cultures established in the present study represent the disease phenotype of schizophrenia as we observed previously in 2D2,10, while providing extra biological insights in terms of cell aggregation and 3D neurite outgrowth (Figs 4A,D and 5). This evidence concerns the gene MAP2 and schizophrenia.