By quantitatively analyzing ptau sites in different brain regions of AD cases we found that most analyzed ptau sites, pSer202/Thr205, pThr231, pSer199, pTyr18 and pSer422, have a very similar allo- and isocortical phosphorylation profile, suggesting that pSer202/Thr205 tau analysis by AT8 antibody could be replaced by any of these ptau sites when using this quantitative histological approach. Here, MAPT is linked to Alzheimer disease.