TNF and hyperandrogenism: To test this hypothesis, in addition to the detection of TNF-α, MDA and serum hormone levels to measure inflammation, OS and hyperandrogenism, respectively, we investigated the number of follicles, the apoptotic index of the follicular cells, SIRT1 and AMPK immunreactivity in the follicles and the ultrastructure of the oocytes and the granulosa-theca cells in an experimental PCOS model.