To determine whether debris-stimulated colon tumor growth was specific to subcutaneous tumors, we also established a debris-stimulated orthotopic colon tumor model in which 1 × 103 living CT26, 9 × 104 5-FU-generated CT26 debris, or the combination of dead and living cells were injected directly into the wall of the cecum in Balb/c mice. The gene discussed is DDX53; the disease is colonic neoplasm.