Subsequently, we found that the autophagy inhibitor 3-MA or URB decreased the autophagosomes accumulation, the expression of beclin-1, and the LC3-II to LC3-I ratio and increased the levels of p62, CTSD, and LAMP1, suggesting that URB may inhibit the lysosomal dysfunction after CCH, which could activate its neuroprotective effects against the abnormal excessive autophagy. The gene discussed is CTSD; the disease is columnar cell hyperplasia of the breast.