When HCC cells were pretreated with the ROCK inhibitor (Y27632 2HCl), RhoA(V14)-induced rescues of cell migration and invasion were blocked (Fig. 4e and f, and Additional file 1: Figure S4c and d, p < 0.01 in Huh-7 and MHCC-97H cells), implying that supervillin-induced increase in HCC cell migration and invasion during hypoxia require RhoA/ROCK signal transduction. This evidence concerns the gene RHOA and hepatocellular carcinoma.