For one thing, AZD1775-reduced PLK1 may contribute to AZD1775-induced DNA damage and apoptosis still presented in WEE1 CRISPR-Cas9 knockout cancer cells [20], in line with reliance of AZD1775’s cytotoxicity on targeting PLK1 and WEE1 in our GC cells (Figs. 1 and 2). Here, PLK1 is linked to gastric cancer.