The high promoter methylation and significant downregulation of IGF2BP1 was observed in all metastatic cell lines including MTLn3, MDA435, MDA231, and 4T1 but slightly in non-metastatic cell lines including MTC in T47D, and the promoter demethylation of IGF2BP1 induced its endogenous expression in metastatic MTLn3 cells, indicating epigenetic modifications could act a role in silencing the IGF2BP1 in metastatic breast tumor cells [54, 57]. Here, IGF2BP1 is linked to medullary thyroid gland carcinoma.