AFP and hepatocellular carcinoma: ROC curve analysis of these results showed that although AFP was not detected in half of patients with HCC, the simultaneous detection of AFP and anti-SF3B1 antibodies can discriminate HCC up to 87.25% with AUC value of 0.9081 (95% CI 0.8635–0.9528, p < 0.0001), which is also superior to anti-SF3B1 antibody biomarker detection only (73.53%; Fig. 6d).