A total of 102 serum samples from HCC patients (Additional file 1: Table S1) and 85 serum samples of healthy control subjects were analyzed, and the reactivity to XC24p11 represented by the difference of antibody reaction between XC24p11-streptavidin (XC24p11-STA) and epitope-free streptavidin (Eph-STA). The gene discussed is EMD; the disease is hepatocellular carcinoma.