After synthesizing the results, including fold change and bioinformatics data, as well as considering the biological functions of these differentially expressed proteins associated with infection and immunity, 4 up-regulated proteins (ACT, AGP1, CFH, and CP) and 4 down-regulated proteins (CDH1, APOCIII, RBP4, and TF) were selected to be validated by western blotting, using the pooled samples in the discovery set (Figure 3). This evidence concerns the gene SERPINA3 and infection.