Such alterations were in accordance with the study from Amaral and coworkers, in which after infecting C57BL/6 mice intracerebrally with DENV-3, the following changes were observed: (i) increased leukocyte rolling and adhesion in brain microvasculature; (ii) tissue evidences of meningoencephalitis, such as perivascular hemorrhages and infiltration of mononuclear cells in brain and cerebellum; (iii) reactive gliosis; and (iv) immunoreactive cells for anti-NS3 in several brain areas47. This evidence concerns the gene KRAS and meningoencephalitis.