To explore a possible role for AMPK in EMT, we initiated our study by investigating the effects of AMPK activation and inhibition on the expression of markers associated with EMT in a variety of cancer cell lines including breast cancer cell lines MCF7, T47D, BT474 and MDA-MB-231, melanoma cell line MDA-MB-435S and lung adenocarcinoma cell line A549. This evidence concerns the gene PRKAA2 and breast cancer.