Further analysis by real-time PCR demonstrated that both S180 tumor and LGT administration also slightly promoted the mRNA expression of Nrf2-target downstream antioxidative genes such as HO-1, NQO1, and GCLC but there was no significant difference, while compatibility with JQC in the ratios (LGT/JQC from 4/1 to 1/4) all remarkably up-regulated the mRNA expression of these target genes compared with LGT alone administration group (all P<0.01) (Figure 2C–E). This evidence concerns the gene GCLC and neoplasm.