In order to analyze the function of GPRC5a in PaCa, GPRC5a was knocked out from the MIA PaCa-2 and TB32047 cell lines with the CRISPR/Cas9 gene editing system, the knockout effect was evaluated by Western blot and sequencing, and the influence of GPRC5a on proliferation and migration ability was analyzed. The gene discussed is GPRC5A; the disease is permanent neonatal diabetes mellitus-pancreatic and cerebellar agenesis syndrome.