RUNX1 and acute myeloid leukemia: This is particularly relevant to genes where mutations may occur at numerous sites rather than in “hot spots” such as RUNX1. A study of 103 patients (median age 69 years) with RUNX1 mutated AML used PCR amplification followed by amplicon sequencing on the Roche 454 platform [56] affording sensitivity of up to 1:800.