ATP2A2 and alkaline phosphatase measurement: Considering the joint participation of SERCA2 and NCX1 in calcium kinetics and the reduction of their expressions in AP associated with calcium overload13, in this study m-RNA expressions of SERCA2 and NCX1 were analyzed in animals submitted to AP by taurocholate with and without pre-treatment with melatonin and DT, isolated and in association.