PLC and RAF small molecule inhibitors were commercially available, and we confirmed that the kinetic curves (captured over pumping cycles) for peptides PLCG1_764_776 and RAF1_332_344 demonstrated increased phosphorylation upon incubation with lysates from GBM cells exposed to low glucose in comparison to standard glucose (Fig. 4). This evidence concerns the gene HSPG2 and glioblastoma.