MBNL1 and myotonic dystrophy type 1: At the protein level, these differences were further enhanced and western blots detected 4–5-fold more MBNL1, and 3–5-fold higher MBNL2 proteins, in DM1 myoblasts after 96 h (Fig. 4a, b, d, e) and 48 h (Supplementary Fig. 6) of antagomiR treatment.