To test whether higher amounts of MBNL1 and MBNL2 mRNA translate into rescue of the Muscleblind-dependent splicing events, we transfected DM1 cells using the optimal conditions determined above and verified improvement of missplicing events including Bridging integrator 1 (BIN1)44, ATPase sarcoplasmic/endoplasmic reticulum Ca2+transporting 1 (ATP2A1)45, Insulin receptor (INSR)46,47, and Piruvate kinase M (PKM)48. This evidence concerns the gene PKM and myotonic dystrophy type 1.