Trizol法提取HCC-827及HCC-827/GR细胞总RNA,利用反转录试剂盒反转录为cDNA,用qRT-PCR法测定ALDH1A1、ALDH1A3、ALDH1B1、ALDH1L1、ALDH7A1、ALDH3A1、ALDH3A2、ALDH3B1和ALDH3B2 mRNA的表达水平,以管家基因GAPDH为内参。. The gene discussed is ALDH3A2; the disease is hepatocellular carcinoma.