In CRC samples known CRC-associated genes including heparan sulfate-glucosamine 3-sulfotransferase 2 (HS3ST2), dickkopf WNT signaling pathway inhibitor 2 (DKK2), tissue factor pathway inhibitor 2 (TFPI2) and syndecan 2 (SDC2) occurred in the top50 significantly hypermethylated 100 base paired regions (p < 0.001), showing elevated promoter DNA methylation levels located within CpG islands. The gene discussed is HS3ST2; the disease is colorectal carcinoma.