TP53 and colorectal carcinoma: DNA methylation alterations were also detected in the mutation hot-spot regions of 12 analyzed CRC-associated genes that are frequently mutated, including TP53, APC, KRAS, BRAF and FBXW7. DNA methylation changes on 100 base pair long analysis windows located on mutation hot-spot regions of TP53, APC, KRAS, BRAF and FBXW7 can be seen in Fig. 2.