Decreasing levels of miR-375, measured by qPCR, with more severe histology were observed also by Bierkens and colleagues on HPV-positive frozen biopsies (6 normal cervical squamous epithelial specimens, 13 CDKN2A-positive HG-CIN, and 9 SCCs) [52]. This evidence concerns the gene CDKN2A and cervical squamous intraepithelial neoplasia.