Use of a supervised learning approach indicates that the serum HCC biomarkers OPN, AFP and DKK1 are excellent diagnostics for HCC in NASH with AUROCs of 0.97, 0.99 and 0.89, respectively, and thresholds of 136 ng/mL, 218 ng/mL and 2.4 ng/mL, respectively. Here, SPP1 is linked to metabolic dysfunction-associated steatohepatitis.