CD163 and penile neoplasm: In addition, to determine the subtype of macrophages infiltrating penile tumors, a fluorescent double staining of CD163 and CD68 was performed (Figures 4A,B) and the majority of cells were found to be CD68+CD163+ both intratumoral and in stromal areas, indicative of M2-polarization of virtually all macrophages in these tumors.