VAFs in cells and tissues were approximately 50% in all cases (I, M, K, O, U, V), as expected, with the exception of KRAS in LoVo (Q and S, approximately 75%), confirming copy number variation of the KRAS MUT allele in the absence of chromosome 12 duplication (see whole genome sequencing data in the Cancer Cell Line Encyclopedia at https://portals.broadinstitute.org/ccle). The gene discussed is KRAS; the disease is cancer.