Further analysis using another mammary epithelial cell line (H184B5F5/M10) co-cultured with fibroblast from both normal mammary tissue adjacent to both ER(-) (Part A in S3 Fig) and ER(+) (Part B in S3 Fig) primary breast cancers was also performed, which revealed similar results as the co-cultured MCF-10A cells. The gene discussed is ESR1; the disease is breast cancer.