Having previously shown a tendency for a higher percentage of Gal-8-positive chondrocytes in MS ≥ 9 than MS ≤ 4 regions and having also reported absence of immunopositivity in specimens of articular cartilage from four osteosarcoma patients, we extended this immunohistochemical analysis by higher patient numbers (15 donors), by including a wide range of degree of cartilage degeneration (from MS 1 to MS 13) and by applying a more detailed approach considering the exact MS of all histological sections analyzed. This evidence concerns the gene LGALS8 and osteosarcoma.