To examine the function of GRAMD1B in JAK/STAT signaling-mediated biological processes such as cell invasion and migration in breast cancer cells, we first assessed the potency of si-Gramd1b, and found that it can efficiently knockdown Gramd1b, as evidenced by the significant reduction of both mRNA (Fig. 2a) and protein levels (Fig. 2b,b’). The gene discussed is GRAMD1B; the disease is breast carcinoma.