Finally, we analyzed the expression of p16, NGFR, WT1 and MME in 37 DIPG patient samples with H3.3K27M mutation and 58 non-brainstem pediatric high-grade glioma (NBS-HGG) samples with wild type H3.3 using the public database (Wu et al., 2014). Here, WT1 is linked to malignant glioma.