To investigate the anti-AD effect of EAH on pro-inflammatory cytokines and chemokines production upon TNF-α/IFN-γ co-stimulation, HaCaT cells were pretreated with EAH for 1 h followed by TNF-α/IFN-γ for 24 h and the supernatant was collected for cytokine level measurement by ELISA (Figure 2B,D). This evidence concerns the gene TNF and Alzheimer disease.