The negative impact of SIRT1 on GH-induced IGF-1 mRNA expression was also confirmed by the same team in HepG2 (human hepatocellular carcinoma cell line) and rat primary hepatocytes with use of SIRT1 inhibitors (sirtinol, nicotinamide) and stimulators (resveratrol, NAD). This evidence concerns the gene SIRT1 and hepatocellular carcinoma.