Co-treatment of the CRC cell lines with brusatol also reduced the IC50 of 5-flurouracil, although drug synergy was achieved at fewer concentrations than noted with irinotecan (Supplementary Figure 4), possibly due to a differential influence of Nrf2 on the metabolism of 5-flurouracil. The gene discussed is NFE2L2; the disease is colorectal carcinoma.