RUNX3 and neoplasm: Methylation of tumor tissue was assessed using five genes that have been associated with the CIMP phenotype: CACNA1G, MLH1, NEUROG1, RUNX3, and SOCS1. Hypermethylation of MLH1 has been associated with development of CRC11, while methylation levels of the other markers have been shown to provide sharply-distinguished high- and low-methylation groups of CRC tumors12.