In order to lend further support to our 32-gene signature, we measured the significance of and concordance with another gene set recently suggested to be essential for effector CD8+ T cell activity for immunotherapy.19 This gene set was identified by a 2CT-CRISPR assay as candidate genes essential for effector CD8+ T cell function and were correlated with cytolytic activity across almost all TCGA cancer types. Here, CD8A is linked to cancer.