Expression of glypican 1 (GPC1) a membrane-bound protein on circulating exosomes of mice and humans with PC can differentiate healthy control and patients with a benign pancreatic disease. Notably, GPC1+ exosomes level correlated with tumor burden and the survival of PC patients before and after the surgery with utter sensitivity and specificity. Additionally, circulating GPC1+ exosomes of PC patients bear KRAS mutations,. Here, KRAS is linked to pachyonychia congenita.